Honeybee Evolution and Behaviour Team (EVOLBEE)

Team members

Researchers

Gérard Arnold, Team Leader, CNRS Research Director. Honeybee behaviour: Impact of the invasive hornet Vespa velutina on honeybees and the ecosystem; the honeybee as a bioindicator.

Lionel Garnery, Lecturer, University of Versailles Saint-Quentin en Yvelines. Evolutionary history of honeybee species, phylogeography, molecular evolution, conservation of genetic and ecoethological diversity in honeybees.

Florence Mougel-Imbert, Lecturer, University Paris XI. Honeybee genetics: characterisation of recombination levels (recombination hotspots after controlled crossing), QTLs, analysis of linkage disequilibrium.

Jean-Christophe Sandoz, CNRS Research Director. Olfactory and visual perception and learning in the honeybee. Sensory and neural circuits involved in pheromone perception in different honeybee species and subspecies. Techniques: in vivo calcium imaging, neuroanatomy, behaviour (appetitive and aversive conditioning, orientation).

Engineers and Technicians

Benoît Lapeyre, CNRS Engineer Assistant. Morphometrical analysis of honeybee populations.

Hélène Legout, CNRS Technician. Characterization of honeybee genetic diversity.

Yves Loublier, INRA Research Engineer. Plant biodiversity analyzed from pollen and nectar gathered by honeybees.

Postdoctoral fellows

Andreas Brandstaetter, NeRF Postdoctoral fellow. Evolution of pheromonal communication in the genus Apis.

Sibyle Moulin, Postdoctoral fellow. Analysis of genetic diversity in French honeybee populations.

Ana-Carolina Roselino, Postdoctorante. Neuroanatomy of the olfactory system of stingless bees.

PhD Students

Florian Bastin, PhD Student. Evolution of sexual communication in honeybees

Bénédicte Bertrand, PhD Student. Role of drones in the genetic diversity of honeybee populations.

Julie Carcaud, PhD Student. Function of the dual olfactory system of honeybees: behavioural and neurophysiological approaches.

Antoine Couto, PhD Student. Neurophysiological and behavioural study of the invasive hornet Vespa velutina.

Pierre Junca, PhD Student. Behavioural and genetic study of aversive and appetitive learning in honeybees.

Master Students

Marie-Anne Wycke, M2R Student. Learning of olfactory mixtures in honeybees.

Previously in the team

Mohamed Alburaki, previously PhD Student, Analysis of honeybee genetic diversity in Syrian honeybee (A. m. syriaca) populations.

Mariangela Arca, PhD Student. Genetic characterization and behavioural study of an invasive hornet species in France: Vespa velutina.

Michel Solignac, previously Professor at University Paris XI, Honeybee genetics.

Team Publications:

2013

• Hatjina, F., Papaefthimiou, C., Charistos, L., Dogaroglu, T., Bouga, M., Emmanouil, C., Arnold, G. (2013). Sublethal doses of imidacloprid decreased size of hypopharyngeal glands and respiratory rhythm of honeybees in vivo. Apidologie , DOI 10.1007/s13592-013-0199-4.


• Lefer, D., Perisse, E., Hourcade, B., Sandoz, J.C. , Devaud, J.M. (2013). Two waves of transcription are required for long-term memory in the honeybee. Learning and Memory, 20, 29-33.


• Monceau, K., Arca, M., Leprêtre, L., Mougel, F., Bonnard, O., Silvain, J.F., Maher, N., Arnold, G., Thiéry, D. (2013). Native prey and invasive predator patterns of foraging activity: The case of the Yellow-Legged Hornet predation at European Honeybee hives. PLoS ONE (in press).


• Mota, T., Gronenberg, W., Giurfa, M., Sandoz, J.C. (2013). Chromatic processing in the anterior optic tubercle of the honeybee brain Journal of Neuroscience, 33, 4-16.

2012

• Arca, M., Capdevielle-Dulac, C., Villemant, C., Mougel, F., Arnold, G., Silvain, J.F. (2011). Development of microsatellite markers for the yellow-legged Asian hornet, Vespa velutina, a major threat for European bees. Conservation Genetic Resources, 4(2), 283-286.


• Arenas, A., Giurfa, M., Sandoz, J.C., Hourcade, B., Devaud, J.M., Farina, W.M. (2012). Early olfactory experience induces structural changes in the primary olfactory centre of an insect brain. European Journal of Neuroscience, 35(5), 682-690.


• Bessoltane, N., Toffano-Nioche, C., Solignac M., Mougel F. (2012). Fine scale analysis of crossover and non-crossover and detection of recombination sequence motifs in the honeybee (Apis mellifera). PLoS ONE, 7(5), e36229, 1-10.


• Carcaud, J., Hill, T., Giurfa, M., Sandoz J.C. (2012). Differential coding by two olfactory subsystems in the honey bee brain. Journal of Neurophysiology, 108, 1106-1121.


• Deisig, N., Kropf, J., Vitecek, S., Pevergne, D., Rouyar, A., Sandoz, J.C., Lucas, P., Gadenne, C., Anton, S., Barrozo, R. (2012). Differential interactions of sex pheromone and plant odour in the olfactory pathway of a male moth. PLoS ONE, 7(3), e33159, 1-10.


• Galizia, C.G., Franke, T., Menzel, R., Sandoz, J.C. (2012). Optical imaging of concealed brain activity using a gold mirror in honeybees. Journal of Insect Physiology , 58(5), 743-749.


• Giurfa, M., Sandoz, J.C. (2012). Invertebrate learning and memory: fifty years of olfactory conditioning of the proboscis extension response in honey bees. Learning and Memory, 19, 54-66.


• Matsumoto, Y., Menzel, R., Sandoz, J.C., Giurfa, M. (2012). Revisiting olfactory classical conditioning of the proboscis extension response in honey bees: a step toward standardized procedures Journal of Neuroscience Methods, 211(1), 159-167.


• Mougel, F., Solignac, M., Vautrin, D., Baudry, E., Ogden, J., Tchapla, A., Schweitz, H., Gilbert, H. (2011). Quantitative traits loci (QTL) involved in body colour, wing morphometry, cuticular hydrocarbons and venom components in honeybee. Apidologie, 43(2), 162-181.


• Papachristoforou, A., Kagiava, A., Papaefthimiou, C., Termentzi, A., Fokialakis, N., Skaltsounis, A.L., Watkins, M., Arnold,G., Theophilidis,G. (2012). The bite of the honeybee: 2-heptanone secreted from honeybee mandibles during a bite acts as a local anaesthetic in insects and mammals. PLoS ONE, 7(10): e47432, 1-11.

2011

• Alburaki, M., Alburaki, A., Legout, H., Moulin, S., Garnery, L. (2011). Mitochondrial structure of Eastern honey bee populations from Syria, Lebanon and Iraq. Apidologie, 42, 628-641.


• Mota, T., Giurfa, M., Sandoz, J.C. (2011). Color modulates olfactory learning in honeybees by an occasion setting mechanism. Learning and Memory, 18, 144-155.


• Mota, T., Roussel, E., Sandoz, J.C., Giurfa, M. (2011). Visual conditioning of the sting extension reflex in harnessed honeybees. Journal of Experimental Biology, 214, 3577-3587.


• Mota, T., Yamagata, N., Giurfa, M., Gronenberg, W., Sandoz, J.C. (2011). Neural organization and visual processing in the anterior optic tubercle of the honeybee brain. Journal of Neuroscience, 31, 11443-11456.


• Papachristoforou, A., Rortais, A., Sueur, J., Arnold, G. (2011). Attack or retreat: contrasted defensive tactics used by Cyprian honeybee colonies under attack from hornets. Behavioural Processes, 86, 236-241.


• Rortais, A., Arnold, G., Alburaki, M., Legout, H., Garnery, L. (2011). Review of the DraI-COI-COII test for the conservation of the black honeybee (Apis mellifera mellifera). Conservation Genetic Resources, 3, 383-391.


• Sandoz, J.C. (2011). Behavioural and neurophysiological study of olfactory perception and learning in honeybees. Frontiers in Systems Neuroscience, 5: 98, 1-20.

2010

• Deisig, N., Giurfa, M., Sandoz, J.C. (2010). Antennal lobe processing increases separability of odor mixture representations in the honeybee. Journal of Neurophysiology, 103, 2185-2194.


• Dupuy F., Josens R., Giurfa M., Sandoz, J.C. (2010). Calcium imaging in the ant Camponotus fellah reveals a conserved odour-similarity space in insects and mammals. BMC Neuroscience, 11:28, 1-13.


• Faille, A., Ribera, I., Deharveng, L., Bourdeau, C., Garnery, L., Queinnec, E., Deuve,T. (2010) A molecular phylogeny shows the single origin of the Pyrenean subterranean Trechini ground beetles (Coleoptera: Carabidae). Molecular Phylogenetics and Evolution, 54,97-106.


• Hourcade, B., Muenz, T.M., Sandoz, J.C., Rössler W., Devaud, J.M. (2010). Long-term memory leads to synaptic reorganization in mushroom bodies: a memory trace in the insect brain? Journal of Neuroscience, 30, 6461-6465.


• Miguel I., Baylac M., Iriondo M., Manzano C., Garnery L., Estonba A. (2010). Both geometric morphometric and microsatellite data consistently support the differentiation of the Apis mellifera M evolutionary branch. Apidologie, DOI; 10.1051/apido/2010048


• Roussel, E., Sandoz, J.C., Giurfa, M. (2010) Searching for learning-dependent changes in the antennal lobe: simultaneous recording of neural activity and aversive olfactory learning in honeybees. Frontiers in Behavioural Neuroscience, 4:155, 1-15.


• Scheiner, R., Arnold, G. (2010). Effects of patriline on gustatory responsiveness and olfactory learning in honey bees. Apidologie, 41, 29–37.

2009

• Arenas, A., Giurfa, M., Farina, W., Sandoz, J.C. (2009). Early olfactory experience modifies neural activity in the antennal lobe of a social insect at the adult stage. European Journal of Neuroscience, 30, 1498-1508.


• Bernadou A., Démares F., Couret-Fauvel T., Sandoz, J.C., Gauthier M. (2009). Effect of fipronil on side-specific antennal
  tactile learning in the honeybee. Journal of Insect Physiology, 55, 1099-1106.


• Carcaud, J., Roussel, E., Giurfa, M., Sandoz, J.C. (2009) Odour aversion after olfactory conditioning of the sting extension reflex in honeybees. Journal of Experimental Biology, 212, 620-626.


• Giurfa, M., Fabre, E., Flaven-Pouchon, J., Groll, H., Oberwallner, B., Vergoz, V , Roussel, E., Sandoz, J.C. (2009). Olfactory conditioning of the sting extension reflex in honeybees: memory dependence on trial number, interstimulus interval, intertrial interval, and protein synthesis. Learning and Memory, 16, 761-765


• Haddad, N., Meixner, M.D., Fuchs, S., Migdadi, H., Garnery, L., Sheppard, W.S. (2009) Mitochondrial DNA support for genetic reserves of Apis mellifera syriaca in Jordan. Journal of Apicultural Research, 48, 19-22.


• Hourcade, B., Perisse, E., Devaud, J.M., Sandoz, J.C. (2009). Long-term olfactory memory shapes the primary olfactory centre of an insect brain. Learning and Memory, 16, 607-615.


• Perisse, E., Raymond-Delpech, V., Néant, I., Matsumoto, Y., Leclerc, C., Moreau, M., Sandoz, J.C. (2009). Early calcium increase triggers olfactory long-term memory formation in honeybees. BMC Biology, 7:30,1-9.


• Rortais, A., Arnold, G., Garnery, L. (2009). Utilisation des marqueurs moléculaires pour la mise en place de conservatoires d'abeilles noires (Apis mellifera mellifera). Mémoires de la Société Entomologique de France, 8, 115-120.


• Roussel, E., Carcaud, J., Sandoz, J.C., Giurfa, M. (2009) Reappraising social insect behavior through aversive responsiveness and learning. PLoS ONE, 4, e4197, 1-9.

Articles in national journals

• Maxim L., Arnold G. (2012). Entre recherche académique et expertise scientifique : des mondes de chercheurs. Hermès, 64, 9-13.
  
• Maxim L., Arnold G. (2012). Comment les conflits d’intérêts peuvent influencer la recherche et l’expertise. Hermès, 64, 48-59.

Book Chapters

• Sandoz, J.C. (2013). Neural correlates of olfactory learning in the primary olfactory center of the honeybee brain: the antennal lobe. In: Invertebrate Learning and Memory, Menzel, R. and Benjamin. P.R. Eds. Elsevier, San Diego (USA), 12 pp.


• Sandoz, J.C. (2011). Olfaction in honeybees: from molecules to behaviour. In: Honeybee Neurobiology and Behavior: A Tribute to Randolf Menzel, Eds: Eisenhardt D., Galizia C.G., Giurfa M., Springer, Berlin, 235-252.


• Behrens, D., Mougel, F., L’Anthoëne, V., Merlin, I., Jaffé, R., Roussel, E.,Titera, D., Giurfa, M., Solignac, M., Moritz, R.F.A. (2011). Beekeeping without chemical treatment? In: Bees in Europe and sustainable Honey Production (BEESHOP): results of a pan-European research network. Eds: Behrends D., Moritz, R.F.A. Nova Science Publishers,


• Sandoz, J.C. (2009). Olfactory plasticity. In: Encyclopedia of Neuroscience, Springer, Heidelberg, 3013-3017.